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Gas Chromatography | working principle and instrumentation lecture

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Gas chromatography lecture - This chromatography lecture explains about the instrumentation, principle of gas chromatography. Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture (the relative amounts of such components can also be determined). In some situations, GC may help in identifying a compound. In preparative chromatography, GC can be used to prepare pure compounds from a mixture. This lecture will explain the principle of gas chromatography and the instrumentation and uses of gas chromatography. In gas chromatography, the mobile phase (or "moving phase") is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen. Helium remains the most commonly used carrier gas in about 90% of instruments although hydrogen is preferred for improved separations.The stationary phase is a microscopic layer of liquid or polymer on an inert solid support, inside a piece of glass or metal tubing called a column (an homage to the fractionating column used in distillation). The instrument used to perform gas chromatography is called a gas chromatograph (or "aerograph", "gas separator"). Gas chromatography is in principle similar to column chromatography (as well as other forms of chromatography, such as HPLC, TLC), but has several notable differences. First, the process of separating the compounds in a mixture is carried out between a liquid stationary phase and a gas mobile phase, whereas in column chromatography the stationary phase is a solid and the mobile phase is a liquid. (Hence the full name of the procedure is "Gas–liquid chromatography", referring to the mobile and stationary phases, respectively.) Second, the column through which the gas phase passes is located in an oven where the temperature of the gas can be controlled, whereas column chromatography (typically) has no such temperature control. Finally, the concentration of a compound in the gas phase is solely a function of the vapor pressure of the gas. Gas chromatography is also similar to fractional distillation, since both processes separate the components of a mixture primarily based on boiling point (or vapor pressure) differences. Gas chromatography is also sometimes known as vapor-phase chromatography (VPC), or gas–liquid partition chromatography (GLPC). These alternative names, as well as their respective abbreviations, are frequently used in scientific literature. Strictly speaking, GLPC is the most correct terminology, and is thus preferred by many authors. In most modern GC-MS systems, computer software is used to draw and integrate peaks, and match MS spectra to library spectra. Gas Chromatography is used extensively in forensic science. Article source: wikipedia I don't own this article written in the description. © goes to original content developers from Wikipedia. For more information, log on to- http://www.shomusbiology.com/ Get Shomu's Biology DVD set here- http://www.shomusbiology.com/dvd-store/ Download the study materials here- http://shomusbiology.com/bio-materials.html Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology- Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching We are social. Find us on different sites here- Our Website – www.shomusbiology.com Facebook page- https://www.facebook.com/ShomusBiology/ Twitter - https://twitter.com/shomusbiology SlideShare- www.slideshare.net/shomusbiology Google plus- https://plus.google.com/113648584982732129198 LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661 Youtube- https://www.youtube.com/user/TheFunsuman Thank you for watching gas chromatography lecture
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Text Comments (522)
Pavithra Venkat (9 days ago)
U r fabulous!!! Wish I had a teacher like you at school as my love and passion for science could have developed and gone further!! I am glad that your videos will help so many people out there including my 13 yr old!! Best wishes and lots of love from England
Pavithra Venkat (3 days ago)
I have subscribed Shomu!! Keep up the great work
Shomu's Biology (9 days ago)
Thank you so much for appreciating my efforts. Glad you liked my lectures. Please subscribe and share
Sam Hernandez (7 hours ago)
This is good, keep this up sir
Peter (19 hours ago)
Excellent teaching. Hats off sir
Shomu's Biology (14 hours ago)
Thank you so much for appreciating my efforts
Kishor Khandre (2 days ago)
Fabulous sir... teaching was awesome 👌
Shomu's Biology (2 days ago)
Thank you. Glad you liked my lectures
Soumya Mishra (7 days ago)
U r genius.
Shomu's Biology (7 days ago)
Thank you
Geeni Chinni (7 days ago)
Sir.. I request you to do video on lc ms sir....
BILIN S BABU (11 days ago)
You are awesome
Shomu's Biology (11 days ago)
Thank you
Russell John Burog (13 days ago)
Lecture is excellent and clear. I have a question. How about components that have the same molecular weight like methylacetylene-propadiene? how are they going to be separated? and also. may I request that you create a video about heart cut technique. thank you
Ania Wo (19 days ago)
Excellent content. Thank you.
Shomu's Biology (19 days ago)
You're welcome
NAIRUTI HEDAMBA (22 days ago)
Bt sir... How to stationary phase work in this type of chromatography?
Aliyahbhat Bhat (23 days ago)
Really enjoy your class.Even tough topics seems so easy the way you teach.
Shomu's Biology (22 days ago)
Thank you very much for appreciating my efforts
Karwan Kamal (23 days ago)
great lecture . we appreciate you for this lecture only i have a question about the (He) -why we use He ? -what happen if we use another gas instead of He?
husnain abbas almani (4 days ago)
The reason to use He is the Difference in TCD value. it is common and best approach to use carrier gas which has far more different TCD values with the product gases
Natasha Chand (26 days ago)
Have a querry. Will organic compound not degenerate using GC?
jalil khan (1 month ago)
Thanku sir... May Allah bless u always... I really undrstand everything what u convey.. like da method!!
Shomu's Biology (1 month ago)
Thanks. Glad to hear that you are getting benefit from the videos
Navdeep jethi (1 month ago)
How to compare these peaks with standards?? When to inject working or references standards??
abdullah sharif (1 month ago)
nice lecture but u didn’t mention column oven....great brother...
Faridul Islam (1 month ago)
excellent explanation of the complex term...so thanks a lot bro from BD
Shomu's Biology (1 month ago)
Thank you. Glad you liked my lectures
I wishing you joy,happiness and success comes in your life.
Shomu's Biology (1 month ago)
Thank you. Glad you liked my lectures
Tithi Das (1 month ago)
It seems too much easy now!
Shomu's Biology (1 month ago)
You're welcome
md ibrahim ebu (1 month ago)
valo laglo
Shomu's Biology (1 month ago)
Dhonyobad
Arju Ali Khan (1 month ago)
I am a student of Horticulture. In recent time, we use for different volatile compounds that are present in horticultural/agronomic crop. Thanks for this wonderful illustration. Thanks for every video where you make biology more and more interesting to me.
Shomu's Biology (1 month ago)
Glad to hear that you are getting benefit from the videos
nanahachi kurenai (1 month ago)
I needed refresher, and this actually gave more depth on how the instrument works. Good video, thanks!
Chandrachur Ghosh (1 month ago)
For me the concept was never been so clear, but sir you have just make it happened. Thank you.
Deeksha Mathew (1 month ago)
thank you sir.
Shomu's Biology (1 month ago)
You're welcome
shabeer ahmed (2 months ago)
Great Class.....Simple, Well Explained and very Informative ..... Superbb..,....
Shomu's Biology (2 months ago)
Thank you. Glad you liked my lectures
GCMS LIKH k to gas chromatography ko explain kr dia,,, it is not good
Fahimeh Mirshekari (2 months ago)
perfect explanation, thanks sir
Shomu's Biology (2 months ago)
You're welcome
Boss Boss Boss Boss (2 months ago)
Super explanation about gc tq so much bro
Baskarla sravani (2 months ago)
Tq so much sir
Shomu's Biology (2 months ago)
You're welcome
Shifa' (2 months ago)
Thank you.
Technical Brainz team (3 months ago)
But sir if you inject polar sample in polar column it will bleed and damage the column
Layali Diab (3 months ago)
الله يرضى عليك ويهديك للإسلام 😍
Mehwish Nawaz (3 months ago)
Sir is it right that elution of compounds is also affected by the temperature or heat As the column is present in oven..
TI Ibrahim (3 months ago)
Thank u sir
Shomu's Biology (3 months ago)
You're welcome
Priyanshu Kapil (3 months ago)
As u told stationary phase will depand upon the sample we are analysing, but if we have no idea about the sample then??
lakshmi shree (3 months ago)
the explanation is very clear. thank u sir
Shomu's Biology (3 months ago)
You're welcome
Ipsita Sahoo (3 months ago)
What if the green coloured are non polar n mod. Volatile n red are polar n highly volatile.. which Will move faster!?
Syful Islam (3 months ago)
Very good lecture.
siddhi tutorial (3 months ago)
Your videos are just....superb and very well explained
Shomu's Biology (3 months ago)
Glad to hear that you are getting benefit from the videos
Bharat Dev (3 months ago)
Excellent way to describe
Shomu's Biology (3 months ago)
Thank you
Nishantanang (3 months ago)
Hydrogen or air ka role to batao
RICHA GAUTAM (3 months ago)
Make this Soo easier to understand..... Thank you for being there... We really want this type of info to clear our doubts... Thank you sir
mukta biswas (3 months ago)
Sir please make a video about Raman spec 🙏 as soon as possible 😨.... it's urgent.
Darji Kandarp (3 months ago)
Tq so much.great job keep it up
Shomu's Biology (3 months ago)
Glad you liked my lectures
Rajashekar Pathi (3 months ago)
you are mind blowing lecturer!!!!!
Shomu's Biology (3 months ago)
Thank you. Glad you liked my lectures
eccentric girl niji (4 months ago)
I have to make a chemistry report for gas chromatography and it sucks... chem sucks. But thanks
Shomu's Biology (4 months ago)
You're welcome
segomotso seggie (4 months ago)
Thanx for da lecture sir...better understandin now
Shomu's Biology (4 months ago)
You're welcome
Amit Basumatary (4 months ago)
YOU ROCKS OUR PROFESSOR SUCKS.......
Shomu's Biology (4 months ago)
Thank you for appreciating my efforts
Ranadip Bera (4 months ago)
Very Nice.. How can calculate the concentration from the peak area of the the sample? Please give a explanation in details.
Shomu's Biology (4 months ago)
I will make a different video on that topic
Israr Khan (4 months ago)
thankssssss a lot for understanding
Shomu's Biology (4 months ago)
You're welcome
Kaviya Kavi (4 months ago)
Thank you for your teaching. It was easily understood.
Grace Cholobesa (4 months ago)
Thank God you explain in English...very easy to understand ....i find it hard to find english tutorials on YouTube
Shomu's Biology (4 months ago)
Glad you liked my lectures
siddaling bhusagond (4 months ago)
Thank you sir
Shomu's Biology (4 months ago)
You're welcome
jackie Aber (4 months ago)
you are the best. i have really understood chromatography today. thank you
Mohamad Kais (4 months ago)
you are professional with GC , your video help me a lot , thank you so much
Shomu's Biology (4 months ago)
You're welcome. Glad you liked my lectures
Sakshi Sardana (4 months ago)
what a explaination sirrr superb thank u
Shomu's Biology (4 months ago)
You're welcome. Glad to hear that you are getting benefit from the videos
sunidhi dhadwal (4 months ago)
What happen if we have: polar+ highly volatile...???
kapil mehta (4 months ago)
what a explain sir👌👌👌👌👌👌 better than university professor great job👌👌👌👌👌👌thank u sir
Neeraj Sirohi (4 months ago)
This vedio is really helpful thanku so much clear my all doubts about GC thanks shomu keep it up
vicmansep (4 months ago)
Excellent explanation! did you describe packed column or capillary one?
Shomu's Biology (4 months ago)
You're welcome
your vines (4 months ago)
Brilliant sir you are awesome its very easy to understand your lecture thanks alot
Zakk Peters (5 months ago)
Very clear explanation, thank you!
Nilantha Jayawardhana (5 months ago)
Well explained. Crystal clear. Thanks 👍
Shomu's Biology (5 months ago)
Thank you. Glad you liked my lectures
umair bin irfan (5 months ago)
I have some questions, If you can help me? What is the appropriate column length and how it affects resolution?
umair bin irfan (5 months ago)
Excellent Video :) Interesting and easily understandable :) Hope you best of luck (y)
Pakhi Gupta (5 months ago)
Thankyou so much Sir for explaining things in the best possible simplest ways!
Subhamoy panda (5 months ago)
Nice
Rehan Merza (5 months ago)
Sir ham solid organic compound ko separate kar sakte h gas chromatography se like phthalicanhydrate
vivek sisodiya (5 months ago)
very easy to understand thank u sir
Priyanka Subash (5 months ago)
Thank you suman battarcharjee😊👍
Shomu's Biology (5 months ago)
You're welcome
Matthew Ludivico (5 months ago)
A very nice, introductory presentation. You might follow up with explanation of stationary phase chemistries.
Shomu's Biology (5 months ago)
Thank you
Nournoby Khan (5 months ago)
clear english......boss
Shomu's Biology (5 months ago)
You're welcome
Viji Amlu (5 months ago)
superbbbbbbb boss
Shomu's Biology (5 months ago)
You're welcome
ali abd (5 months ago)
Thank you a lot
Shomu's Biology (5 months ago)
You're welcome
Omkar Dongare (5 months ago)
Thank you !
Shomu's Biology (5 months ago)
You're welcome
Prabhat Tripathi (5 months ago)
Very nice sir.. I could't understand it in my university...
Shomu's Biology (5 months ago)
You're welcome
Kirsti Gilmore (5 months ago)
I love your lecture!!!
Shomu's Biology (5 months ago)
Thank you. Glad you liked my lectures
Paninga Muiliya (5 months ago)
I love this. It is clear and simple. Very comprehensive. Thanks.
gopika vrindha (5 months ago)
systamatic approach to topics makes it easy to absorb...thanku
javed tamboli (6 months ago)
Crystal clear explanation
Shomu's Biology (6 months ago)
Thank you
Mohd Owais (6 months ago)
Excellent
Shomu's Biology (6 months ago)
Thank you. Glad you liked my lectures
Smriti Chettri (6 months ago)
Very well explained sir thank you so much
Shomu's Biology (6 months ago)
Glad to Know that
Narender Upadhyay (6 months ago)
great job....easy to understand....
Narender Upadhyay (6 months ago)
my self lecturer in chemistry my students also like you . my video related to chemistry uploded as upadhyay chemistry
Shomu's Biology (6 months ago)
Thank you. Glad you liked my lectures
malhar malushte (6 months ago)
hi, i am trying to measure the mixture of exhaust gases from my experiemtn. the gases in the mixutre could be O2, CO, CO2, CH4, C3H8, N2. out of this my columns only detect O2, CO, CO2, N2. In such case how to calibrate the GC system. i am not aware of the procedure or what actually happens or how to accomodate for the gases which dont show up in the chrpmatogram for eg CH4 or C3H8 as column doesnt detect them. could you please guide?
Naman Gupta (6 months ago)
u r great
Engr.Imtiaz Ahmad (5 months ago)
excellent teaching style thank you sir
Shomu's Biology (6 months ago)
Thank you
ANAGHA THOMAS (6 months ago)
Thank you so much Sir.. really helpful and easy to understand.. This helped me a lot
Shomu's Biology (6 months ago)
Glad it helped
AAKASH GUPTA (6 months ago)
Sir, You need to train our Professors, also can you please provide me with the write up for Pyrolysis Gas Chromatography , I will be very thankful to you.
mrphysh (6 months ago)
your subtitles are out of sync.
akshu bhardwaj (6 months ago)
Thank you so much
박bina (6 months ago)
If my professors were all like u, i wouldve got As only. Thank u a million !
Half Asleep (6 months ago)
Thanks.
Rajesh Kshirsagar (6 months ago)
Thank you Mr. Shomu it was interesting ! well i would like to understand one thing that you have mentioned packed bed columns and Capillary column but i think you have not given any idea about capillary column so can you explain well about capillary column ?
BaaYo (6 months ago)
Watch Gas Chromatography video in Hindi On "BaaYo" Channel.
wolverine (6 months ago)
you are god at this bro... keep up the good work
Sweet Ghar (7 months ago)
Sir kia ap urdu m explain kr sakty hn please
Jackson Robson (7 months ago)
hello shomus biology why is it not possible to achieve reproducible injections?
Unknown Mystery (7 months ago)
Nice vidio , can you make the gas chromatography system used in oil drill well site in mudlogging lab,
anand gaja (7 months ago)
thank u sir. i clearly understand
Atish Mohapatra (7 months ago)
Thanks
subrahmanyam gollapalli (7 months ago)
why use inert gas what about other gases

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